ZBTB33 Knockout cell line (22RV1)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

ZBTB33 Knockout cell line (22RV1) Order

Catalog#

YKO-H691

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ZBTB33 Knockout cell line (22RV1)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Gene Name

ZBTB33

Gene ID

10009

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% RPMI-1640+10% FBS

Target region

Knockout region: Exon 3 (based on transcript ZBTB33-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:22RV1			STR Info (Cell bank) Cell Line:22RV1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	12	13.0
D13S317	9	12		9	12
D7S820	9	10	11.0	10	11	9.0
D16S539	12	12		12	12
VWA	15	21		15	21
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	10	11		10	11

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZBTB33 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 22RV1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZBTB33 gene knockout ZBTB33 Knockout cell line (22RV1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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ZBTB33 Knockout cell line (22RV1)

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ZBTB33 Knockout cell line (22RV1) Order

Detailed Product Information

Instruction

Related Popular Products

Wild-type Cell Lines

Human Prostate Carcinoma Cell Line(22RV1) More details Order

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit More details Order

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium More details Order

KO Cell Lines

ZBTB33 Knockout cell line (Hela) inquiry More details

ZBTB33 Knockout cell line (HCT 116) inquiry More details

ZBTB33 Knockout cell line (HEK293) inquiry More details

Related Popular Products

Wild-type Cell Lines

Human Prostate Carcinoma Cell Line(22RV1)

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

EZ-cryo™ cell freezing medium

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KO Cell Lines

ZBTB33 Knockout cell line (Hela)

ZBTB33 Knockout cell line (HCT 116)

ZBTB33 Knockout cell line (HEK293)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Human Prostate Carcinoma Cell Line(22RV1)
More details Order

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More details Order

EZ-cryo™ Cell Freezing Medium
More details Order

ZBTB33 Knockout cell line (Hela)
inquiry More details

ZBTB33 Knockout cell line (HCT 116)
inquiry More details

ZBTB33 Knockout cell line (HEK293)
inquiry More details